Health standard & # 32; Analysis of preservatives in cosmetics

Preservatives in cosmetics is in the process of cosmetics production, use and preservation, in order to prevent bacterial contamination and join an additive. China's regulations on hygienic standards for cosmetics with limited material, the use of a kind of preservative limited. Due to various nutrients, cosmetics added these nutrients are microbial proliferation and reproduction of culture medium, so in the production, use and preservation of cosmetics, is likely to get bacterial pollution and make the product produce odor or change the appearance, to inhibit the growth of bacteria breeding, must add some preservatives in products. Most of the preservative has a certain toxicity to human body. Different preservatives toxic level, should be determined according to the intensity of toxicity usage. Some strong toxic preservatives have been banned from use, such as 2 chlorophenol, bromine chlorine phenol, etc. Preservatives are divided into the following categories: acids, esters, phenols, halides and quaternary ammonium salts, etc. Currently the most used in cosmetic preservative is low toxicity and good sterilization effect of hydroxy benzoic acid esters. The determination methods of preservatives have absorbance spectrophotometry, titration method, gas chromatography, thin layer chromatography (TLC), high performance liquid chromatography. Absorbance spectrophotometry for raw material analysis, for example - Amino anti than Lin colorimetric method for quantitative containing hydroxyl compound. Titration method is mainly used for quantitative quaternary ammonium salts preservatives. Gas chromatography for determination of the polar material such as containing hydroxyl and carboxyl, general need methyl silane, derived using gc-ms after qualitative and quantitative. Therefore, in the preservative detection. Thin layer chromatography (TLC) is one of the most widely used method of preservatives in the analysis. In the biggest characteristic is to qualitatively by means of a variety of components, and does not require special equipment, easy to promote. High performance liquid chromatography (HPLC) method is currently the most widely used method. Because of the preservative is mostly macromolecular organic matter, and ultraviolet absorption characteristics, the most suitable for using high performance liquid chromatography (HPLC) analysis. The method has advantages of simple operation and high sensitivity.